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大鼠誘導(dǎo)型一氧化氮合成酶(NOS2/iNOS)酶聯(lián)免疫吸附檢測試劑盒

ELK1503

規(guī)格：	價(jià)格：
48T	￥1960.00
96T	￥2800.00

下載說明 ①

Overview 文獻(xiàn)

Product name:	Rat NOS2/iNOS(NitricOxide Synthase 2, Inducible) ELISA Kit
Reactivity:	Rat
Alternative Names:	NOS2A; INOS; HEP-NOS; I-NOS; Hepatocytes Oxide Synthase; Peptidyl-cysteine S-nitrosylase NOS2; NOS2; Nitric Oxide Synthase 2, Inducible
Assay Type:	Sandwich
Sensitivity:	0.62 ng/mL
Standard:	100 ng/mL
Detection Range:	1.57-100 ng/mL
Sample Type:	tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Enzyme & Kinase;Metabolic pathway;Tumor immunity;Cardiovascular biology;
Uniprot ID:	Q06518
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat NOS2/iNOS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NOS2/iNOS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat NOS2/iNOS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NOS2/iNOS in the samples is then determined by comparing the OD of the samples to the standard curve.

Journal of Neuroinflammation

Regulation of the p75 neurotrophin receptor attenuates neuroinflammation and stimulates hippocampal neurogenesis in experimental Streptococcus pneumoniae meningitis

日期：2021-11-02 IF：8.322
International Immunopharmacology

Combined sulforaphane and β-sitosterol mitigate olanzapine-induced metabolic disorders in rats: Insights on FOXO, PI3K/AKT, JAK/STAT3, and MAPK signaling pathways

日期：2024-10-25 IF：4.8

標(biāo)準(zhǔn)曲線

Concentration (ng/mL)	OD	Corrected OD
100.00	1.979	1.883
50.00	1.562	1.466
25.00	1.029	0.933
12.50	0.885	0.789
6.25	0.561	0.465
3.13	0.357	0.261
1.57	0.268	0.172
0.00	0.096	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant NOS2/iNOS and the recovery rates were calculated by comparing the measured value to the expected amount of NOS2/iNOS in samples.

Matrix	Recovery range	Average
serum(n=5)	81-97%	89%
EDTA plasma(n=5)	84-97%	90%
Heparin plasma(n=5)	82-94%	88%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NOS2/iNOS and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.