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人高遷移率族蛋白B1(HMGB-1)酶聯(lián)免疫吸附檢測試劑盒

ELK1438

規(guī)格：	價格：
48T	￥1960.00
96T	￥2800.00

下載說明 ①

Overview

Product name:	Human HMGB-1(High Mobility Group Protein B1) ELISA Kit
Reactivity:	Human
Alternative Names:	HMGB1; HMG3; SBP1; Sulfoglucuronyl Carbohydrate Binding Protein; Amphoterin; High Mobility Group Box 1 Protein; HMG1; High Mobility Group Protein 1
Assay Type:	Sandwich
Sensitivity:	26.9 pg/mL
Standard:	4000 pg/mL
Detection Range:	62.5-4000 pg/mL
Sample Type:	serum, plasma and other biological fluids
Assay Length:	3.5h
Research Area:	Infection immunity;
Uniprot ID:	P09429
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HMGB-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HMGB-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HMGB-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HMGB-1 in the samples is then determined by comparing the OD of the samples to the standard curve.

標準曲線

Concentration (pg/mL)	OD	Corrected OD
4000.00	1.961	1.868
2000.00	1.436	1.343
1000.00	1.196	1.103
500.00	0.841	0.748
250.00	0.614	0.521
125.00	0.415	0.322
62.50	0.225	0.132
0.00	0.093	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant HMGB-1 and the recovery rates were calculated by comparing the measured value to the expected amount of HMGB-1 in samples.

Matrix	Recovery range	Average
serum(n=5)	80-97%	88%
EDTA plasma(n=5)	85-101%	93%
Heparin plasma(n=5)	83-97%	90%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HMGB-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.