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日期：2025-01-17
IF：11.4標(biāo)準(zhǔn)曲線
| Concentration (ng/mL) | OD | Corrected OD |
|---|---|---|
| 10.00 | 2.266 | 2.198 |
| 5.00 | 1.593 | 1.525 |
| 2.50 | 1.183 | 1.115 |
| 1.25 | 0.752 | 0.684 |
| 0.63 | 0.553 | 0.485 |
| 0.32 | 0.325 | 0.257 |
| 0.16 | 0.225 | 0.157 |
| 0.00 | 0.068 | 0.000 |
精密度
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
回收率
Matrices listed below were spiked with certain level of recombinant HMGB-1 and the recovery rates were calculated by comparing the measured value to the expected amount of HMGB-1 in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=5) | 79-97% | 88% |
| EDTA plasma(n=5) | 80-95% | 87% |
| Heparin plasma(n=5) | 84-98% | 91% |
線性
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HMGB-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 87-96% | 85-97% | 85-94% | 87-96% |
| EDTA plasma(n=5) | 92-101% | 82-98% | 89-97% | 85-93% |
| Heparin plasma(n=5) | 86-96% | 93-104% | 89-97% | 85-94% |
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