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人生長調(diào)節(jié)致癌基因a(GROa/CXCL1)酶聯(lián)免疫吸附檢測試劑盒

ELK1024

規(guī)格：	價格：
48T	￥1680.00
96T	￥2400.00

下載說明 ①

Overview 文獻

Product name:	Human GROa/CXCL1(Growth Regulated Oncogene Alpha) ELISA Kit
Reactivity:	Human
Alternative Names:	CXCL1; GRO1; GROa; GRO-A; MGSA-A; NAP3; SCYB1; Chemokine C-X-C-Motif Ligand 1; Melanoma Growth Stimulating Activity Alpha; Fibroblast Secretory Protein; Neutrophil Activating Protein 3
Assay Type:	Sandwich
Sensitivity:	6.1 pg/mL
Standard:	1000 pg/mL
Detection Range:	15.63-1000 pg/mL
Sample Type:	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Cytokine;Tumor immunity;Infection immunity;
Uniprot ID:	P09341
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GROa/CXCL1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GROa/CXCL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GROa/CXCL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GROa/CXCL1 in the samples is then determined by comparing the OD of the samples to the standard curve.

WOUND REPAIR AND REGENERATION

Foamy macrophages potentially inhibit tuberculous wound healing by inhibiting the TLRs/NF-κB signalling pathway

日期：2022-04-06 IF：3.617

標準曲線

Concentration (pg/mL)	OD	Corrected OD
1000.00	2.168	2.027
500.00	1.841	1.700
250.00	1.384	1.243
125.00	0.969	0.828
62.50	0.649	0.508
31.25	0.481	0.340
15.63	0.315	0.174
0.00	0.141	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant GROa/CXCL1 and the recovery rates were calculated by comparing the measured value to the expected amount of GROa/CXCL1 in samples.

Matrix	Recovery range	Average
serum(n=5)	82-97%	89%
EDTA plasma(n=5)	95-107%	101%
Heparin plasma(n=5)	81-95%	88%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GROa/CXCL1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.