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下載說明 ①Overview
標準曲線
| Concentration (ng/mL) | OD | Corrected OD |
|---|---|---|
| 5000.00 | 0.189 | |
| 2500.00 | 0.397 | |
| 1250.00 | 0.583 | |
| 625.00 | 0.833 | |
| 312.50 | 1.157 | |
| 156.25 | 1.583 | |
| 78.13 | 1.715 | |
| 0.00 | 2.211 |
精密度
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
回收率
Matrices listed below were spiked with certain level of recombinant 25-OH-D and the recovery rates were calculated by comparing the measured value to the expected amount of 25-OH-D in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=5) | 85-97% | 91% |
| EDTA plasma(n=5) | 91-105% | 98% |
| Heparin plasma(n=5) | 95-107% | 101% |
線性
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 25-OH-D and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 85-107% | 84-98% | 95-103% | 86-98% |
| EDTA plasma(n=5) | 86-93% | 91-105% | 87-98% | 99-102% |
| Heparin plasma(n=5) | 85-97% | 87-96% | 86-98% | 91-103% |
產品成分表
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